Preparation and the use of halopropargylated cyclic quaternary ammonium compounds as antimicrobial agents

ABSTRACT

Antimicrobial compounds of the formula    &lt;IMAGE&gt;  (I)  wherein R is selected from (A)(C1to C20) straight or branched alkyl, optionally substituted with hydroxy, halogen, alkoxy, phenoxy, or -C(=O)R&#39; wherein R&#39; is selected from C1-C20)alkyl, (C1-C20)alkoxy, phenoxy, or NHR&#39;&#39; wherein R&#39;&#39; is (C1-C6)alkyl or phenyl; (B) benzyl; (C) phenethyl; (D) cyanoethyl; (E) 2-cyanoethyl; (F) propargyl; and (G) allyl; X=halogen (chlorine, bromine, or iodine), phosphate, acetate, benzoate, citrate, tartrate, alkyl- or aryl-sulfonate, alkylsulfate; and   &lt;IMAGE&gt;s at least one nitrogen atom optionally substituted with one or more substituents selected from (C1-C3)alkyl, halo, and carboxyl, said ring optionally containing one or two hetreoatoms in addition to said one nitrogen atom, selected from sulfur, oxygen, and a second nitrogen; Processes for inhibiting microbial growth, and compositions suitable therefore are also disclosed.

This is a divisional of U.S. patent application Ser. No. 07/948,367, filed Sep. 21, 1992, now U.S. Pat. No. 5,266,567 which is a continuation-in-part of U.S. patent application Ser. No. 07/782,358, filed Oct. 24, 1991, now abandoned.

I. BACKGROUND OF THE INVENTION

A. Field of the Invention

This invention relates to novel quaternary ammonium antimicrobial compounds and their use.

B. Description of the Prior Art Quaternary ammonium compounds having the general formula (A): ##STR3## wherein R₁, R₂, and R₃ are all substituted or unsubstituted carbon chains were disclosed in U.S. Pat. No. 4,521,412 as having antifungal and antibacterial activity.

Tropium quaternary ammonium compounds having the general structure (B): ##STR4##

wherein X=chlorine were evaluated for hypotensive activity in J. Org. Chem., 24, 1607 (1959). No antimicrobial activity was mentioned in this paper.

The chloride salt and saccharinate salt of tropium (C) (i.e. X=saccharine) were mentioned in U.S. Pat. No. 4,521,412. ##STR5##

Cyclic quaternary ammonium compounds which do not contain an iodopropargyl group have been disclosed in J. Pharmacol. Exptl. Therap., 121, p.347 (1957), where piperazine mono-quaternary salts were shown to have activity against mouse pinworm.

U.S. Pat. No. 4,233,055 discloses substances of the following general structure (D) for inhibiting the growth of plants and for promoting the ripening and abscission of fruit. ##STR6##

R=alkyl, alkenyl, alkynyl

Cyclic quaternary ammonium compounds have been claimed to be used as surface active agents, bactericides, biocides, algicides or fungicides, as exemplified by the following: ##STR7##

DD-278-268-A, Fungicides ##STR8##

U.S. Pat. No. 4,472,412 (1984), Fungicides ##STR9##

U.S. Pat. No. 3,506,666 (1970), For the control of algae, bacteria and fungi. ##STR10##

U.S. Pat. No. 2,971,006 (1961), claimed to be used as surface active agents, bactericides, biocides, and particularly as fungicities.

Japanese Patent 67-026603B discloses a method of producing 3-halo-2-propynylamines of the formula

(R₁)(R₂)N--CH₂ CC--X

in which R₁ and R₂ may be joined to form a saturated cyclic 6-membered piperidine or morpholine ring.

II. SUMMARY OF THE INVENTION

Many of the antimicrobials of the prior art are ineffective against gram negative bacteria, have toxicity, and/or environmental problems. It is an object of this invention to provide antimicrobial compounds which have excellent activity against a broad spectrum of fungi and bacteria, and have favorable toxicological and environmental profiles.

Their objects and others are achieved by the present invention which comprises compounds having antimicrobial activity having the formula: ##STR11## wherein

R is selected from (A) (C₁ to C₂₀) straight or branched alkyl, optionally substituted with hydroxy, halogen, alkoxy, phenoxy, or --C(═O)R' wherein R' is selected from (C₁ -C₂₀)alkyl, (C₁ -C₂₀)akloxy, phenoxy, or NHR" wherein R" is (C₁ -C₆)alkyl or phenyl; (B) benzyl; (C) phenethyl; (D) cyanoethyl; (E) 2-cyanoethyl; (F) propargyl; and (G) allyl;

X=halogen (chlorine, bromine, or iodine), phosphate, acetate, benzoate, citrate, tartate, alkyl- or aryl-sulfonate, alkylsulfate; and ##STR12##

represents a 3-to 7-membered ring that contains at least one nitrogen atom optionally substituted with one or more substitutents selected from (C₁ -C₃)alkyl, halo, and carboxyl, said ring optionally containing one or two hetreroatoms in addition to said one nitrogen atom, selected from sulfur, oxygen, and a second nitrogen.

III. DETAILED DESCRIPTION OF THE INVENTION AND THE PREFERRED EMBODIMENTS

Preferred compounds of the invention are those wherein R═C₁₀ to C₁₆ alkyl, ##STR13## is pyrrolidinium, piperidinium, morpholinium, dimethylmorpholinium, or tetramethylmorpholinium, and X is bezenesulfonate or methane sulfonate. Preferred compounds include the following:

1.1-(2-hydroxyethyl)-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

2. 1-(12-hydroxydodecyl)-l-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

3. 1-(8-hydroxyoctyl)-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

4. 1-(7-hydroxyheptyl)-1-(3-iodopropargyl)-pyrrolidinium 4-methybenzenesulfonate

5. 1-(9-hydroxynonyl)-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

6. 1-tetradecyl-1-(3-iodopropargyt)-pyrrolidinium 4-methylbenzenesulfonate

7. 1-hexadecyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

8. 1-tridecyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

9. 1-butyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

10. 1-pentadecyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

11. 1-decyl-1-(3-iodopropargyl-pyrrolidinium 4-methylbenzenesulfonate

12. 1-octyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

13. 1-dodecyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

14. 1-hexyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

15. 1 -undecyl -1 -(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

16. t-octadecyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

17. -methyl-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate

18. 1-(2-hydroxyethyl)-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate

19. 1 -(11-hydroxyundecyl)-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate

20. 1-(12-hydroxydodecyl)-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate

21. 1-(9-hydroxynonyl)-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate

22. 1-(2-ethoxycarbonylethyl)-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate

23. 1-(8-hydroxyoctyl)-1-(3-iodopropargyl )-1-piperidinium 4-methylbenzenesulfonate

24. 1-(7-hydroxyheptyl)-1-(3-iodopropargyl)-piperidinium- 4methylbenzenesulfonate

25.1-(tetradecyl)-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate

26. 1-(hexadecyl)-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate

27. 1 -(12-hydroxydodecyl)-1 -(3-iodopropargyl)-hexamethyleneiminium 4-methylbenzenesulfonate

28. 4-tetradecyl-4-(3-iodopropargyl)-morpholinium 4-methylbenzenesulfonate

29. 4-methyl-4-(3-iodopropargl)-morpholinium 4-methylbenzenesulfonate

30. 1-dodecyl-1-(3-iodopropargyl)-pyrrolidinium benzenesulfonate

31. 1-tridecyl-1-(3-iodopropargyl)-pyrrolidinium benzenesulfonate

32. 1-hexadecyl-1-(3-iodopropargy)-pyrrolidinium benzenesulfonate

33. 1-pentadecyl-1-(3-iodopropargyl)-pyrrolidinium benzenesulfonate

34. 1 -tetradecyl-1-(3-iodopropargyl)-pyrrolidinium benzenesulfonate

35. 1-hexadecyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonate

36. 1-tridecyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonate

37. 1-dodecyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonate

38. 1-pentadecyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonate

39. 1-tetradecyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonate

40. 1-hexadecyl-1-(3-iodopropargyt)-morpholinium methanesulfonate

41. 4-tetradecyl-4-(3-iodoprolyargyl)-morpholinium benzenesulfonate

42. 4-tetradecyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

43. 4-tetradecyl-4-(3-iodopropargyl)-2,6-dimethylmorpholinium 4-methylbenzenesulfonate

44. 1-hexadecyl-1-(3-iodopropargyl)-hexamethyleneiminium 4-methylbenzenesulfonate

45. 1-tetradecyl-1-(3-iodopropargyl)-hexamethyleneiminium 4-methylbenzenesulfonate

46. 4-hexadecyl-4-(3-iodopropargyl)-2,6-dimethylmorpholinium 4-methylbenzenesulfonate

47. 4-hexadecyl-4-(3-iodopropargyl)-2,6-dimethylmorpholinium benzenesulfonate

48. 4-hexadecyl-4-(3-iodopropargyl)-2,6-dimethylmorpholinium methanesulfonate

49. 4-hexadecyl-4-(3-iodopropargyl)-morpholinium 4-methylbenzenesulfonate

50. 4-hexadecyl-4-(3-iodopropargyl)-morpholinium benzenesulfonate

51. 1-(tetradecyl)-1-(3-iodopropargyl)-2-methylpiperidinium benzenesulfonate

52. 1-(tetradecyl)-1-(3-iodopropargyl)-4-methylpiperidinium benzenesulfonate

53. 1-(tetradecyl)-1-(3-iodopropargyl)-1,2,3,6-tetrahydropyridinium benzenesulfonate

54. 1-(tetradecyl)-1-(3-iodopropargyl)-2-methylpiperidinium 4methylbenzenesulfonate

55. 1-(tetradecyl)-1-(3-iodopropargyl)-2-methylpiperidinium methanesulfonate

56. 1-(tetradecyl)-1-(3-iodopropargyl)-imidazolinium benzenesulfonate

57. 4-(tetradecyl)-4-(3-iodopropargyl)-thiomorpholinium benzenesulfonate

58.3-(tetradecyt)-3-(3-iodopropargyl)-imidazolidinium benzenesulfonate

59. 1-tetradecyl-1-(3-iodopropargyl)-piperidinium benzenesulfonate

60. 1-hexadecyl-1-(3-iodopropargyl)-piperidinium benzenesulfonate

61. 1-hexadecyl-1-(3-iodopropargyl)-piperidinium methanesulfonate

62. 1-tetradecyl-1-(3-iodopropargyl)-piperidinium methanesulfonate

63. 1-(dodecyloxycarbonyl)methyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

64. 1-(dodecyloxycarbonyl)methyl-1-(3-iodopropargyl)-pyrrolidinium benzenesulfonate

65. 1-(decyioxycarbonyl)methyl-1-(3-iodopropargyl)-pyrrolidinium 4methylbenzenesulfonate

66.4-(decyioxycarbonyl)methyl-4-(3-iodopropargyl)-morpholiniurn 4methylbenzenesulfonate

67. 4-(decyloxycarbonyl)methyl-4-(3-iodopropargyl)-morpholinium benzenesulfonate

68. 4-(dodecyloxycarbonyl)methyl-4-(3-iodopropargyl)-morpholinium 4-methylbenzenesulfonate

69. 1-tetradecyl-1-(3-iodopropargyl)-pyrrolidinium bromide

70. 1-(decyloxycarbonyl)methyl-1-(3-iodopropargyt)-pyrrolidinium benzenesulfonate

71. 4-tetradecyl-4-(3-iodopropargyl)-morpholinium bromide

72. 4-tetradecyl-4-(3-iodopropargyl)-rnorpholinium iodide

73. 4-benzyl-4-(3-iodopropargyi )-morpholinium 4-methylbenzenesulfonate

74. 4-benzyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

75. 1-(dodecyloxycarbonyl)methyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonate

76. 4-(dodecyloxycarbonyl)methyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

77. 1-n-butyl-1-(3-iodopropargyl)-pyrrolidinium bromide

78. 1-decyl-1-(3-iodopropargyl )-pyrrolidinium bromide

79. 1-dodecyl-1-(3-iodopropargyl)-pyrrolidinium bromide

80. 1-hexadecyl-1-(3-iodopropargyl)-pyrrolidinium bromide

81. 4-[5-(hexyloxycarbonyl)lpentyl-4-(3-iodopropargy1)-morpholinium 4-methylbenzenesulfonate

82. 1-[5-(hexyloxycarbonyl)]pentyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

83. 4-[5-(hexyloxycarbonyl)]pentyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

84. 1-[5-(hexyloxycarbonyl)]pentyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonate

85. 1-tetradecyl-1-(3-iodoproparygyl)-piperidinium n-butylsulfonate

86. 4-tetradecyl-4-(3-iodopropargyl)-morpholinium n-butylsulfonate

87. 1.-tetradecyl-1-(3-iodopropargyl)-pyrrolidinium n-butylsulfonate

88. 4-pentadecyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

89. 4-tridecyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

90. 4-dodecyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

91. 4-decyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

92. 4-n-octyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

93. 4-n-butyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

94. 4-ethyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

95. 4-[2-(decyloxycarbonyl)]ethyl-4-(3-iodopropargyl)-morpholinium methanesulfonate

96. 4-[2-(decyloxycarbonyl)]ethyl-4-)3-iodopropargyl)-morpholinium 4-methanesulfonate

97. 1-[2-(decyloxycarbonyl)]ethyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate

98. l-[2-(decyloxycarbonyl)]ethyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonte

99. 4-n-butyl-4-(3-iodopropargyl)-morpholinium bromide

100. 4-hexadecyl-4-(3-iodopropargyl)-morpholinium bromide

101. 4-dodecyl-4-(3-iodopropargyl)-morpholinium bromide

Table 1 shows the structures and the physical data of these representative compounds.

    __________________________________________________________________________     Structures and Physical Data                                                    ##STR14##                           (I)                                        No.                                                                               ##STR15##                                                                             R        X            Melting Point                                 __________________________________________________________________________         ##STR16##                                                                            C.sub.2 H.sub.4OH                                                                        ##STR17##   136-138° C.                             2                                                                                  ##STR18##                                                                            C.sub.12 H.sub.24OH                                                                      ##STR19##   65-70° C.                               3                                                                                  ##STR20##                                                                            C.sub.8 H.sub.16OH                                                                       ##STR21##   61-67° C.                               4                                                                                  ##STR22##                                                                            C.sub.7 H.sub.14 OH                                                                      ##STR23##   oil                                            5                                                                                  ##STR24##                                                                            C.sub.9 H.sub.18OH                                                                       ##STR25##   89-92° C.                               6                                                                                  ##STR26##                                                                            C.sub.14 H.sub.29 -n                                                                     ##STR27##   141-143° C.                             7                                                                                  ##STR28##                                                                            C.sub.16 H.sub.33 -n                                                                     ##STR29##   139-144° C.                             8                                                                                  ##STR30##                                                                            C.sub.13 H.sub.27 -n                                                                     ##STR31##   140-142° C.                             9                                                                                  ##STR32##                                                                            C.sub.4 H.sub.9 -n                                                                       ##STR33##   140-143° C.                             10                                                                                 ##STR34##                                                                            C.sub.15 H.sub.31 -n                                                                     ##STR35##   143-144° C.                             11                                                                                 ##STR36##                                                                            C.sub.10 H.sub.21 -n                                                                     ##STR37##   137-138° C.                             12                                                                                 ##STR38##                                                                            C.sub.8 H.sub.17 -n                                                                      ##STR39##   136-138° C.                             13                                                                                 ##STR40##                                                                            C.sub.12 H.sub.25 -n                                                                     ##STR41##   140-141° C.                             14                                                                                 ##STR42##                                                                            C.sub.6 H.sub.13 -n                                                                      ##STR43##   122-124° C.                             15                                                                                 ##STR44##                                                                            C.sub.1 H.sub.23 -n                                                                      ##STR45##   140-141° C.                             16                                                                                 ##STR46##                                                                            C.sub.81 H.sub.37 -n                                                                     ##STR47##   138-141° C.                             17                                                                                 ##STR48##                                                                            CH.sub.3                                                                                 ##STR49##   174-178°  C.                            18                                                                                 ##STR50##                                                                            C.sub.2 H.sub.4OH                                                                        ##STR51##   109-112° C.                             19                                                                                 ##STR52##                                                                            C.sub.11 H.sub.22OH                                                                      ##STR53##   89-93° C.                               20                                                                                 ##STR54##                                                                            C.sub.12 H.sub.24OH                                                                      ##STR55##   91-95° C.                               21                                                                                 ##STR56##                                                                            C.sub.9 H.sub.18OH                                                                       ##STR57##   93-98° C.                               22                                                                                 ##STR58##                                                                            C.sub.2 H.sub.4CO.sub.2 Et                                                               ##STR59##   95-99° C.                               23                                                                                 ##STR60##                                                                            C.sub.8 H.sub.16OH                                                                       ##STR61##   129-135° C.                             24                                                                                 ##STR62##                                                                            C.sub.7 H.sub.14OH                                                                       ##STR63##   100-108° C.                             25                                                                                 ##STR64##                                                                            C.sub.14 H.sub.29 -n                                                                     ##STR65##   140-144° C.                             26                                                                                 ##STR66##                                                                            C.sub.16 H.sub.33 -n                                                                     ##STR67##   148-150° C.                             27                                                                                 ##STR68##                                                                            C.sub.12 H.sub.24OH                                                                      ##STR69##   75-79° C.                               28                                                                                 ##STR70##                                                                            C.sub.14 H.sub.29 -n                                                                     ##STR71##   153-154° C.                             29                                                                                 ##STR72##                                                                            CH.sub.3                                                                                 ##STR73##   130-137° C.                             30                                                                                 ##STR74##                                                                            C.sub.12 H.sub.25 -n                                                                     ##STR75##   95-97° C.                               31                                                                                 ##STR76##                                                                            C.sub.13 H.sub.27 -n                                                                     ##STR77##    95-100° C.                             32                                                                                 ##STR78##                                                                            C.sub.16 H.sub.33 -n                                                                     ##STR79##   110-111° C.                             33                                                                                 ##STR80##                                                                            C.sub.15 H.sub.31 -n                                                                     ##STR81##   107-109° C.                             34                                                                                 ##STR82##                                                                            C.sub.14 H.sub.29 -n                                                                     ##STR83##   104-106° C.                             35                                                                                 ##STR84##                                                                            C.sub.16 H.sub.33 -n                                                                    OSO.sub.2.CH.sub.3                                                                          113-117° C.                             36                                                                                 ##STR85##                                                                            C.sub.13 H.sub.27 -n                                                                    OSO.sub.2.CH.sub.3                                                                          100-105° C.                             37                                                                                 ##STR86##                                                                            C.sub.12 H.sub.25 -n                                                                    OSO.sub.2.CH.sub.3                                                                          108-112° C.                             38                                                                                 ##STR87##                                                                            C.sub.15 H.sub.31 -n                                                                    OSO.sub.2.CH.sub.3                                                                          113-118° C.                             39                                                                                 ##STR88##                                                                            C.sub.14 H.sub. 29 -n                                                                   OSO.sub.2.CH.sub.3                                                                          85-90° C.                               40                                                                                 ##STR89##                                                                            C.sub.16 H.sub.33 -n                                                                    OSO.sub.2.CH.sub.3                                                                          111-114° C.                             41                                                                                 ##STR90##                                                                            C.sub.14 H.sub.29 -n                                                                     ##STR91##   134-136° C.                             42                                                                                 ##STR92##                                                                            C.sub.14 H.sub.29 -n                                                                    OSO.sub.2.CH.sub.3                                                                          108-112° C.                             43                                                                                 ##STR93##                                                                            C.sub.14 H.sub.29 -n                                                                     ##STR94##   105-109° C.                             44                                                                                 ##STR95##                                                                            C.sub.16 H.sub.33 -n                                                                     ##STR96##   80-85° C.                               45                                                                                 ##STR97##                                                                            C.sub.14 H.sub.29 -n                                                                     ##STR98##   60-65°  C.                              46                                                                                 ##STR99##                                                                            C.sub.16 H.sub.33 -n                                                                     ##STR100##  glassy                                         47                                                                                 ##STR101##                                                                           C.sub.16 H.sub.33 -n                                                                     ##STR102##  glassy                                         48                                                                                 ##STR103##                                                                           C.sub.16 H.sub.33 -n                                                                    OSO.sub.2.CH.sub.3                                                                          glassy                                         49                                                                                 ##STR104##                                                                           C.sub.16 H.sub.33 -n                                                                     ##STR105##  152-154° C.                             50                                                                                 ##STR106##                                                                           C.sub.16 H.sub.33 -n                                                                     ##STR107##  134-136° C.                             51                                                                                 ##STR108##                                                                           C.sub.14 H.sub.29 -n                                                                     ##STR109##  viscous oil                                    52                                                                                 ##STR110##                                                                           C.sub.14 H.sub.29 -n                                                                     ##STR111##  viscous oil                                    53                                                                                 ##STR112##                                                                           C.sub.14 H.sub.29 -n                                                                     ##STR113##  90-94° C.                               54                                                                                 ##STR114##                                                                           C.sub.14 H.sub.29 -n                                                                     ##STR115##  viscous oil                                    55                                                                                 ##STR116##                                                                           C.sub.14 H.sub.29 -n                                                                    OSO.sub.2.CH.sub.3                                                                          viscous oil                                    56                                                                                 ##STR117##                                                                           C.sub.14 H.sub.29 -n                                                                     ##STR118##  116-120° C.                             57                                                                                 ##STR119##                                                                           C.sub.14 H.sub.29 -n                                                                     ##STR120##  173-177° C.                             58                                                                                 ##STR121##                                                                           C.sub.14 H.sub.29 -n                                                                     ##STR122##  viscous oil                                    59                                                                                 ##STR123##                                                                           C.sub.14 H.sub.29 -n                                                                     ##STR124##  128-130° C.                             60                                                                                 ##STR125##                                                                           C.sub.16 H.sub.33 -n                                                                     ##STR126##  136-139° C.                             61                                                                                 ##STR127##                                                                           C.sub.16 H.sub.33 -n                                                                    OSO.sub.2.CH.sub.3                                                                          semi-solid                                     62                                                                                 ##STR128##                                                                           C.sub.14 H.sub.29 -n                                                                    OSO.sub.2.CH.sub.3                                                                          semi-solid                                     63                                                                                 ##STR129##                                                                           CH.sub.2 COOC.sub.12 H.sub.25                                                            ##STR130##  104-106° C.                             64                                                                                 ##STR131##                                                                           CH.sub.2 COOC.sub.12 H.sub.25                                                            ##STR132##  81-83° C.                               65                                                                                 ##STR133##                                                                           CH.sub.2 COOC.sub.10 H.sub.21                                                            ##STR134##  108-109° C.                             66                                                                                 ##STR135##                                                                           CH.sub.2 COOC.sub.10 H.sub.21                                                            ##STR136##  semi-solid                                     67                                                                                 ##STR137##                                                                           CH.sub.2 COOC.sub.10 H.sub.21                                                            ##STR138##  semi-solid                                     68                                                                                 ##STR139##                                                                           CH.sub.2 COOC.sub.12 H.sub.25                                                            ##STR140##  semi-solid                                     69                                                                                 ##STR141##                                                                           C.sub.14 H.sub.29 -n                                                                    Br           semi-solid                                     70                                                                                 ##STR142##                                                                           CH.sub.2 COOC.sub.10 H.sub.21                                                            ##STR143##  80-82° C.                               71                                                                                 ##STR144##                                                                           C.sub.14 H.sub.29 -n                                                                    Br           141-144° C.                             72                                                                                 ##STR145##                                                                           C.sub.14 H.sub.29 -n                                                                    I            85-88° C.                               73                                                                                 ##STR146##                                                                           Benzyl                                                                                   ##STR147##  161-165° C.                             74                                                                                 ##STR148##                                                                           Benzyl   OSO.sub.2.CH.sub.3                                                                          168-170° C.                             75                                                                                 ##STR149##                                                                           CH.sub.2 COOC.sub.12 H.sub.25                                                           OSO.sub.2.CH.sub.3                                                                          50-53° C.                               76                                                                                 ##STR150##                                                                           CH.sub.2 COOC.sub.12 H.sub.25                                                           OSO.sub.2.CH.sub.3                                                                          semi-solid                                     77                                                                                 ##STR151##                                                                           C.sub.4 H.sub.9 -n                                                                      Br           169-172° C.                             78                                                                                 ##STR152##                                                                           C.sub.10 H.sub.21 -n                                                                    Br           118-121° C.                             79                                                                                 ##STR153##                                                                           C.sub.12 H.sub.25 -n                                                                    Br           semi-solid                                     80                                                                                 ##STR154##                                                                           C.sub.16 H.sub.33 -n                                                                    Br           70-73° C.                               81                                                                                 ##STR155##                                                                           (CH.sub.2).sub.5 COOC.sub.6 H.sub.13                                                     ##STR156##  124-126° C.                             82                                                                                 ##STR157##                                                                           (CH.sub.2).sub.5 COOC.sub.6 H.sub.13                                                     ##STR158##  75-77° C.                               83                                                                                 ##STR159##                                                                           (CH.sub.2).sub.5 COOC.sub.6 H.sub.13                                                    OSO.sub.2.CH.sub.3                                                                          semi-solid                                     84                                                                                 ##STR160##                                                                           (CH.sub.2).sub.5 COOC.sub.6 H.sub.13                                                    OSO.sub.2.CH.sub.3                                                                          semi-solid                                     85                                                                                 ##STR161##                                                                           C.sub.14 H.sub.29 -n                                                                    OSO.sub.2 C.sub.4 H.sub.9 -n                                                                145-150° C.                             86                                                                                 ##STR162##                                                                           C.sub.14 H.sub.29 -n                                                                    OSO.sub.2 C.sub.4 H.sub.9 -n                                                                140-143° C.                             87                                                                                 ##STR163##                                                                           C.sub.14 H.sub.29 -n                                                                    OSO.sub.2 C.sub.4 H.sub.9 -n                                                                semi-solid                                     88                                                                                 ##STR164##                                                                           C.sub.15 H.sub.31 -n                                                                    OSO.sub.2.CH.sub.3                                                                          111-113° C.                             89                                                                                 ##STR165##                                                                           C.sub.13 H.sub.27 -n                                                                    OSO.sub. 2.CH.sub.3                                                                         110-113° C.                             90                                                                                 ##STR166##                                                                           C.sub.12 H.sub.25 -n                                                                    OSO.sub.2.CH.sub.3                                                                          glassy                                         91                                                                                 ##STR167##                                                                           C.sub.10 H.sub.21 -n                                                                    OSO.sub.2.CH.sub.3                                                                          semi-solid                                     92                                                                                 ##STR168##                                                                           C.sub.8 H.sub.17 -n                                                                     OSO.sub.2.CH.sub.3                                                                          semi-solid                                     93                                                                                 ##STR169##                                                                           C.sub.4 H.sub.9 -n                                                                      OSO.sub.2.CH.sub.3                                                                          64-65° C.                               94                                                                                 ##STR170##                                                                           C.sub.2 H.sub.5                                                                         OSO.sub.2.CH.sub.3                                                                          155-159° C.                             95                                                                                 ##STR171##                                                                           (CH.sub.2).sub.2 CO.sub.2 C.sub.10 H.sub.21                                              ##STR172##  semi-solid                                     96                                                                                 ##STR173##                                                                           (CH.sub.2).sub. 2 CO.sub.2 C.sub.10 H.sub.21                                            OSO.sub.2.CH.sub.3                                                                          semi-solid                                     97                                                                                 ##STR174##                                                                           (CH.sub.2).sub.2 CO.sub.2 C.sub.10 H.sub.21                                              ##STR175##  75-78° C.                               98                                                                                 ##STR176##                                                                           (CH.sub.2).sub.2 CO.sub.2 C.sub.10 H.sub.21                                             OSO.sub.2.CH.sub.3                                                                          semi-solid                                     99                                                                                 ##STR177##                                                                           C.sub.4 H.sub.9 -n                                                                      Br           190-191° C.                             100                                                                                ##STR178##                                                                           C.sub.16 H.sub.33 -n                                                                    Br           semi-solid                                     101                                                                                ##STR179##                                                                           C.sub.12 H.sub.25 -n                                                                    Br           semi-solid                                     __________________________________________________________________________

Compounds of the invention can be prepared by the following reaction: ##STR180##

Generally, a tertiary cyclic amine of the formula (II) can be reacted with an iodopropargyl compound of the formula (III), in which X represents halogen or a sulfonate group, in the presence of a suitable solvent at temperature between 0° and 100° C.

The compounds of formula (II) and formula (III) are generally commercially available or can be prepared by known methods in the literature. Examples of compounds of formula (II) are 1-methylpiperidine, 1-butylpyrrolidine, 1-(2-hydroxyethyl)-piperidine. Examples of compounds of formula (III) are 3-iodopropargyl-1-chloride, 3-iodopropargyl-1-bromide, and 3-iodopropargyl-1-(4-methylbenzene)sulfonate.

Alternatively, the compounds of the invention can be prepared by the following reaction: ##STR181##

wherein X' is either halogen, alkyl sulfate, alkyl sufonate, or aryl sulfonate.

The following examples illustrate a few embodiments of the invention.

EXAMPLES EXAMPLE 1 Preparation of Iodopropargyl Methanesulfonate ##STR182##

To the solution of 3-iodopropargyl alcohol (5 g, 27.5 mmol) in methylene chloride (150 mL) at 0° C. under nitrogen was added triethylamine (2.8 g, 27.5 mmol). To the above solution, methanesulfonyl chloride (3.2 g, 27.5 mmol) was added dropwise with stirring (magnetic stirrer). The reaction mixture was stirred at 0° to 5° C. for 2 hrs. The reaction mixture was washed with 0.5N HCl (50 mL), diluted sodium bicarbonate (50 mL), water (3×50 mL), and brine. The organic phase was dried over MgSO₄ and filtered. The filtrate was concentrated on a rotary evaporator to afford 5.5 g (yield: 76.9%) of iodopropargyl methanesulfonate as a tan oil. An ¹ H NMR spectrum (CDCl₃) showed the desired compound.

EXAMPLE 2 Preparation of Iodopropargyl 4-Methylbenzenesulfonate ##STR183##

Into a 500 mL 4-neck round bottom flask, equipped with a magnetic stirrer and an addition funnel, in an ice bath were placed iodopropargyl alcohol (10 g, 54.9 mmol) in THF (20 mL) and sodium hydroxide (3.4 g, 84.6 mmol) in water (20 mL) To the above cold mixture was added a solution of p-toluenesulfonyl chloride (10.4 g, 54.95 mmol) in THF (20 mL) from an addition funnel. The reaction mixture was stirred for 2 hrs. below 5° C. The reaction mixture was then poured into ice water and the precipitate was collected by suction-filtration and washed with water. After drying under vacuum, the precipitate afforded 17.2 g (yield: 93.4%) of iodopropargyl 4methylbenzenesulfonate as an off-white solid, mp=75°-78 ° C. An NMR spectrum showed the desired compound.

EXAMPLE 3 Preparation of 1-Tetradecyl-1-(3-Iodopropargyl)-Pyrrolidinium 4-Methylbenzenesulfonate (Compound 6) ##STR184##

In a 250-mL round bottom flask, equipped with a magnetic stirrer, a heating mantle and reflux condenser, were placed 1-bromotetradecane (10 g, 36 mmol), acetonitrile (100 mL) and pyrrolidine (5.1 g, 72 mmol). The reaction mixture was refluxed for 3 hrs. The reaction mixture was cooled to room temperature and was concentrated on a rotary evaporator to a residue. Water and ethyl acetate were added. The organic phase was separated and washed with saturated sodium chloride solution, dried over anhydrous magnesium sulfate. After filtration, the solvent was evaporated to dryness affording 7.2 g (75% yield) of 1-tetradecylpyrrolidine, as a light yellow semi-solid. This intermediate was subjected to the next step without further purification.

In a 250-mL round bottom flask, equipped with magnetic stirrer, heating mantle, and reflux condenser, were placed 1-tetradecylpyrrolidine (2 g, 7.49 mmol), acetone (20 mL), and iodopropargyl 4-methylbenzenesulfonate (2.6 g, 7.76 mmol). The reaction mixture was stirred at room temperature for 5 hrs. The precipitated product was collected by suction-filtration and washed with ether. After drying under vacuum, a white solid of 1-tetradecyl-1-(3-iodopropargyl)pyrrolidinium 4-methylbenzenesulfonate was obtained, 2.1 g (47% yield). mp=141°-143° C. An NN4R spectrum showed the desired compound. An elemental analysis was also performed:

Elemental Analysis of:

    ______________________________________                                                 % C    % H    % N       % S  % I                                       ______________________________________                                         Calculated                                                                               55.71    7.68   2.29    5.31 21.02                                   Found     55.89    7.93   2.32    5.25 20.73                                   ______________________________________                                    

EXAMPLE 4 Preparation of 1-Methyl-1-(3-Iodopropargyl)-Piperidinium 4-Methylbenzenesulfonate (Compound No-17) ##STR185##

In a 250-mL round bottom flask equipped with a magnetic stirrer were placed 1-methylpiperidine (0.3 g, 3.02 mmol) and ether (10 mL). To the above solution was added iodopropargyl 4-methylbenzenesulfonate (1 g, 2.97 mmol). The mixture was stirred at room temperature for 24 hrs. The white solid was collected by suction-filtration, washed with ether and dried in air affording 0.9 g (68.4% yield), m.p.=174°-178 ° C. An NMR spectrum showed the desired structure and the elemental analysis showed the following:

    ______________________________________                                                  % C        % H     % I                                                ______________________________________                                         Calculated 44.1%        5.20%   29.1%                                          Found      44.2%        5.13%   29.0%                                          ______________________________________                                    

EXAMPLE 5 Preparation of 4-Tetradecyl-4-(3-Iodopropargyl)Morpholidinium 4-Methylbenzenesulfonate (Compound No-28) ##STR186##

In a 250-mL round bottom flask, equipped with a magnetic stirrer, a heating mantle and reflux condenser, were placed 1-bromotetradecane (15.9 g, 57.3 mmol), acetonitrile (75 mL), and morpholine (10 g, 114.7 mmol). The reaction mixture was refluxed for 4 hrs. The reaction mixture was cooled to room temperature and was concentrated to a residue on a rotary evaporator. Water and ethyl acetate were added. The organic phase was separated and washed with saturated sodium chloride solution, and then dried over anhydrous magnesium sulfate. After filtration, the solvent was evaporated to dryness affording 15.2 g (94% yield) of 4-tetradecylmorpholine as a tan oil. This intermediate was subjected to the next step without further purification.

In a 250-mL round bottom flask, equipped with magnetic stirrer, heating mantle, and reflux condenser, were placed 4-tetradecylmorpholine (1.7 g, 6 mmol), dry acetone (2.5 mL), and iodopropargyl 4methylbenzenesulfonate (2 g, 6 mmol). The reaction mixture was stirred at room temperature for 40 hrs. The solvent was then evaporated to a residue. The residue was made a slurry with ether and the resultant white suspension was suction-filtered, washed with ether and dried under vacuum, affording 2 g (55.5% yield) of 4-tetradecyl-4-(3-iodopropargyl)-morpholidiniurn 4-methylbenzenesulfonate as a white solid. mp=153°-154 ° C. An NMR spectrum showed the desired compound.

EXAMPLE 6 Biological Activity

Biocidal (bactericidal and fungicidal) evaluations were carried out.

A minimum inhibitory concentration (MIC) value is obtained using a broth, two-fold serial dilution test performed as follows: A stock solution or dispersion of the test compound, typically at a concentration of 1%, is made in a 5:3:2 solvent solution of acetone. methanol, and water. A volume of the stock solution is dispensed into culture media to give an initial starting test concentration of 500 ppm compound.

When the test is ready to be done, each vessel in the dilution series, except the first vessel, contains an equal volume of compound free broth. The first vessel contains twice the volume of broth with the starting concentration of test compound. One half of the broth from the first vessel is transferred to the second vessel- After being mixed, one half the resulting volume is removed from the second vessel and transferred to the third vessel. The entire cycle is repeated sufficiently to give a series of concentrations amounting to 500, 250, 125, 63, 31, 16, 8, and 4, 2, 1, 0.5, 0.25 ppm respectively.

Each vessel is then inoculated with a cell suspension of the appropriate test organism. Bacteria are grown in broth, fungi on agar slants for a time and at a temperature appropriate to the species being tested, and algae are a mixture of green algae and blue-green bacteria grown in a nutrient media. At the end of the growth period, in the case of bacteria, the broth is vortexed to disperse the cells.

In the case of fungi, the spores are harvested by pipetting water onto the slant and dislodging the spores with a sterile loop. The cell/spore suspension is standardized by controlling incubation time, temperature, and the volume of the diluent. The suspension is then used to inoculate the vessels containing the broth compound.

The vessels are then incubated at the appropriate temperature. After the incubation, the vessels are examined for growth/no growth. The minimum inhibitory concentration (MIC) is defined as the lowest concentration of compound that results in complete inhibition of growth of the test organism.

The organisms tested to demonstrate biocidal activity are shorten in the following table:

                  TABLE 2A                                                         ______________________________________                                         MICROORGANISMS USED IN THE BIOCIDES TESTS                                      Name           GRAM     ATCC    Abbreviation used                              ______________________________________                                         BACTERIA                                                                       1. Pseudomonas aeruginosa                                                                     (-)      15442   Psae                                           2. Staphylococcus aureus                                                                      (+)       6538   Saur                                           3. Escherichia coli                                                                           (-)      11229   Ecol                                           FUNGUS                                                                         4. Aspergillus niger     6275   Anig                                           ______________________________________                                    

The result of minimum inhibitory concentration (MIC) tests of compounds of this invention is shown in Table 2.

                                      TABLE 2                                      __________________________________________________________________________     Biocidal MIC Results                                                           MIC (ppm)                                                                            (G-)     (G-)     (G+)     (fungus)                                      Bacteria:                                                                            Psae     Ecol     Saur     Anig                                          Media:                                                                               M9G TSB  M9G TSB  M9G TSB  TSB                                           __________________________________________________________________________     Cpd No                                                                          1    >250                                                                               125  --  >250 16  32   >250                                           2    >250                                                                               >250 --  250  32  32   32                                             3    >500                                                                               >500 --  >500 500 >500 >500                                           4    >500                                                                               >500 >500                                                                               500  500 500  250                                            5    >500                                                                               >500 >500                                                                               >500 63  63   250                                            6    4   4    4   4    0.5 0.5  <0.25                                          7    4   8    4   2    <0.25                                                                              <0.25                                                                               1                                              8    16  16   2   4    0.5 1    1                                              9    500 >500 500 250  250 250  500                                           10    8   250  4   32   <0.25                                                                              1    <0.25                                         11    250 500  16  32   2   16   4                                             12    500 >500 32  63   2   16   16                                            13    32  250  32  32   1   16   16                                            14    500 500  250 125  16  16   250                                           15    32  32   1   2    0.25                                                                               0.25 16                                            16    500 500  16  63   1   1    32                                            17    250 125  --  250  32  32   125                                           18    250 125  --  250  16  32   250                                           19    250 250  --  32   2   16   32                                            20    >250                                                                               >250 --  250  8   63   125                                           21    >250                                                                               >250 --  >250 >250                                                                               >250 63                                            22    >250                                                                               >250 --  >250 >250                                                                               >250 16                                            23    >500                                                                               >500 --  >500 63  250  >500                                          24    >500                                                                               >500 --  >500 63  500  >500                                          25    8   2    2   16   0.25                                                                               0.25 8                                             26    4   16   4   4    0.25                                                                               0.25 16                                            27    >250                                                                               >250 --  250  16  32   32                                            28    4   4    1   2    0.25                                                                               0.25 8                                             29    500 500  250 125  500 500  500                                           30    63  63   2   16   1   1    1                                             31    32  32   1   16   0.5 <0.25                                                                               1                                             32    32  125  4   16   <0.25                                                                              <0.25                                                                               <0.25                                         33    8   32   1   2    <0.25                                                                              <0.25                                                                               <0.25                                         34    2   2    0.25                                                                               0.25 <0.25                                                                              <0.25                                                                               0.25                                          35    >500                                                                               >500 4   63   0.5 0.25 4                                             36    32  63   2   8    <0.25                                                                              <0.25                                                                               1                                             37    63  125  2   16   0.5 0.5  0.25                                          38    32  63   1   8    <0.25                                                                              <0.25                                                                               0.5                                           39    63  63   1   2    <0.25                                                                              <0.25                                                                               0.5                                           40    4   4    0.5 <0.25                                                                               <0.25                                                                              0.5  <0.25                                         41    2   4    <0.25                                                                              <0.25                                                                               <0.25                                                                              0.5  2                                             42    1   4    <0.25                                                                              <0.25                                                                               <0.25                                                                              0.5  1                                             43    2   4    <0.25                                                                              0.5  <0.25                                                                              0.5  1                                             44    8   8    8   16   8   4    8                                             45    4   4    <0.25                                                                              <0.25                                                                               <0.25                                                                              <0.25                                                                               1                                             46    16  16   8   8    <0.25                                                                              <0.25                                                                               2                                             47    8   8    2   2    <0.25                                                                              <0.25                                                                               0.5                                           48    8   8    2        <0.25                                                                              0.5  1                                             49    8   8    1   2    <0.25                                                                              0.5  1                                             50    8   8    1   1    <0.25                                                                              0.5  0.5                                           51    16  16   4   2    <0.25                                                                              <0.25                                                                               0.5                                           52    16  16   8   2    <0.25                                                                              <0.25                                                                               <0.25                                         53    8   16   2   1    <0.25                                                                              <0.25                                                                               <0.25                                         54    250 125  8   16   1   8    1                                             55    125 125  4   16   1   8    1                                             56    8   63   <0.25                                                                              <0.25                                                                               <0.25                                                                              <0.25                                                                               <0.25                                         57    0.5 4    <0.25                                                                              0.5  <0.25                                                                              <0.25                                                                               0.5                                           58    125 >500 4   8    0.5 4    8                                             59    32  125  8   16   4   4    8                                             60    63  250  2   16   1   2    16                                            61    16  125  8   8    2   2    8                                             62    16  63   2   4    0.25                                                                               0.25 0.25                                          63    64  125  2   4    2   4    2                                             64    32  32   1   4    0.25                                                                               0.25 8                                             65    64  125  2   4    2   1    16                                            66    64  250  2   8    0.5 8    125                                           67    125 250  2   8    0.5 8    125                                           68    32  64   2   8    0.5 2    16                                            69    8   32   1   4    0.25                                                                               0.25 4                                             70    125 250  2   8    1   2    32                                            71    8   32   1   4    0.25                                                                               0.25 4                                             72    16  64   0.5 4    0.25                                                                               0.25 4                                             73    >500                                                                               >500 125 125  125 250  125                                           74    >500                                                                               >500 64  125  125 250  64                                            75    32  125  2   8    0.25                                                                               2    8                                             76    64  125  2   8    0.25                                                                               2    32                                            77    >500                                                                               >500 64  64   250 250  >500                                          78    250 500  2   4    0.25                                                                               2    8                                             79    32  64   1   4    0.25                                                                               1    2                                             80    16  125  2   8    0.5 0.5  1                                             81    >500                                                                               >500 64  64   4   16   125                                           82    >500                                                                               >500 32  64   2   8    125                                           83    >500                                                                               >500 16  32   4   8    125                                           84    >500                                                                               >500 16  32   2   8    32                                            85    8   32   1   4    0.25                                                                               0.25 4                                             86    8   32   2   8    0.5 1    8                                             87    16  64   2   4    0.25                                                                               0.25 4                                             88    8   32   2   4    0.25                                                                               0.25 4                                             89    16  64   2   8    1   2    8                                             90    16  125  2   4    2   4    16                                            91    250 >500 4   8    1   8    32                                            92    >500                                                                               >500 32  64   8   16   125                                           93    >500                                                                               >500 125 125  >500                                                                               >500 500                                           94    >500                                                                               >500 125 125  500 >500 >500                                          95    500 500  16  64   32  64   32                                            96    500 >500 8   64   32  64   16                                            97    250 500  16  32   8   16   16                                            98    500 500  16  32   8   32   16                                            __________________________________________________________________________

From Table 2, the most active compounds are those with R=C₁₀ to C₁₆. Furthermore, the cyclic quaternary ammonium compounds which contain an iodopropargyl group, such as those in this invention, are surprising in that they have excellent activity against Gram negative bacteria, such as Pseudomonas aeruginosa or Escherichia coli, when compared with the corresponding cyclic quaternary, ammonium compounds which do not contain an iodopropargyl group.

EXAMPLE 7 Comparative

Table 3 shows the comparison from MIC test results of several representative iodopropargylated cyclic quaternary ammonium compounds of the invention and their corresponding non-iodopropargylated analogues (compound number followed by "A").

The last compound is also a comparative compound and is the tropiumbenzyl sulfonate quat from U.S. Pat. No. 4,521,412, identified as "Bayer analogue."

                                      TABLE 3                                      __________________________________________________________________________     Comparison of Iodopropargylated- and                                           Non-iodopropargylate-Quats                                                     MIC (ppm)                                                                            (G-)     (G-)     (G+)     (fungus)                                      Bacteria:                                                                            Psae     Ecol     Saur     Anig                                          Media:                                                                               M9G TSB  M9G TSB  M9G TSB  TSB                                           __________________________________________________________________________     Cpd No                                                                         6     4   4    4   4    0.5 0.5  <0.25                                         6A    125 250  125 250  <0.25                                                                              <0.25                                                                               <0.25                                         34    2   2    0.25                                                                               0.25 <0.25                                                                              <0.25                                                                               0.25                                          34A   125 250  16  32   1   0.25 16                                            17    250 125  --  250  32  32   125                                           17A   >500                                                                               >500 >500                                                                               >500 >500                                                                               >500 >500                                          Bayer 63  125  15  63   32  125  --                                            Analogue                                                                       __________________________________________________________________________

EXAMPLE 8 Comparative

Table 4 shows the comparison of MIC test results of three compounds of the present invention, 1-(tetradecyl)-1-(3-iodopropargyl)-piperidinium 4-methylbenzenesulfonate, 4-tetradecyl-4-(3-iodopropargyl )-morpholinium benzenesulfonate, and 4-tetradecyl-4-(3-iodopropargyl)-morpholinium methanesulfonate (Compounds 25, 41 and 42, respectively) with the two most c;osely related compounds from Japanese Patent 67-026603B, N-(3-iodo-2-propynyl)-piperidine and N-(3-iodo-2-propynyl)-morpholine (Compounds J1 and J2 respectively). These Japanese compounds are the iodopropargyl amines corresponding to the quaternary ammonium compounds of the present invention.

These five compounds were tested side-by-side against six organisms, Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneurnoniae (K. pneumoniae), Enterobacter aerogenes (E. aerogenes), Proteus mirabilis (P. mirabilis), Staphylococcus aureus (S. aureus), and Aspergillus niger (A. niger). The MIC tests were performed as in example 6.

                  TABLE 4                                                          ______________________________________                                         MIC Values (ppm)                                                                        Invention Compounds                                                                         Japanese Compounds                                       Organism   #25     #41    #42   J1      J2                                     ______________________________________                                         P. aeruginosa                                                                             8       8      16    >500    >500                                   K. Pneumoniae                                                                             4       4      4     125     500                                    E. Aerogenes                                                                              1       2      1      32     250                                    P. mirabilis                                                                              4       4      4     125     500                                    S. aureau  0.5     0.5    1     125     250                                    A. niger   8       8      8      16      8                                     ______________________________________                                    

As can be seen from the data, the quaternary ammonium compounds of the present invention are surprisingly and significantly more active against the organisms tested than those of the Japanese reference.

The compounds of the invention can be used to inhibit the growth of microbes by introducing a microbicidally effective amount of one or more of said compounds onto, into, or at a locus subject to microbial attack. Loci such as wood, paint, adhesive, glue, paper, pulp/paper slurries, textile, leather, plastics, cardboard, lubricants, cosmetics, food, caulking, feed, and industrial cooling water can be protected.

The amount of compound suitable is about 5 to about 300 ppm based on weight of said locus. Generally, the microbicide is applied in a carrier such as water, solvent, or the like.

While the invention has been described in detail, various modifications and alterations should become readily apparent to those skilled in the art without departing from the spirit and scope of the invention. 

I claim:
 1. Compounds having antimicrobial activity having the formula: ##STR187## wherein R is C₁ to C₅) alkyl substituted with --C(═O)R' wherein R' is (C₆ -C₁₂) alkoxy;X=chlorine bromine, iodine, phosphate, acetate, benzoate, citrate, tartrate, alkyl- or aryl-sulfonate, or alkylsulfate; and ##STR188## represents a 5- to 7-membered ring selected from the group consisting of pyrrolidinium, piperidinium, morpholinium, hexamethyleneiminium, tetrahydropyridinium, immidazolinium, thiormorpholinium, and thiazonlidinium, optionally substituted with one or more (C₁ -C₃)alkyl groups.
 2. Compound according to claim 1 selected from the group consisting of1-(dodecyloxycarbonyl)methyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate 1-(dodecyloxycarbonyl)methyl-1-(3-iodopropargyl)-pyrrolidinium benzenesulfonate 1-(decyioxycarbonyl)methyl-1-(3-iodopropargyl)-pyrrolidinium 4methylbenzenesulfonate 4-(decyioxycarbonyl)methyl-4-(3-iodopropargyl)-morpholiniurn 4methylbenzenesulfonate 4-(decyloxycarbonyl)methyl-4-(3-iodopropargyl)-morpholinium benzenesulfonate 4-(dodecyloxycarbonyl)methyl-4-(3-iodopropargyl)-morpholinium 4-methylbenzenesulfonate 1(decyloxycarbonyl)methyl-1-(3-iodopropargyl)-pyrrolidinium benzenesulfonate 1-(dodecyloxycarbonyl)methyl-1-(3iodopropargyl)-pyrrolidinium methanesulfonate 4-(dodecyloxycarbonyl)methyl-4-(3-iodopropargyl)-morpholinium methanesulfonate 4-[5-(hexyloxycarbonyl)]pentyl-4-(3-iodopropargyl)-morpholinium 4-methylbenzenesulfonate 1-[5-(hexyloxycarbonyl)]pentyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate 4-[5-(hexyloxycarbonyl)]pentyl-4-(3-iodopropargyl)-morpholinium methanesulfonate 4-[2-decyloxycarbonyl)]ethyl-4-(3-iodopropargyl-morpholinium 4-methylbenzenesulfonate 4-[2-(decyloxycarbonyl)]ethyl-1-(3-iodopropargyl)-morpholinium-4-methanesulfonate 1-[2-(decyloxycarbonyl]ethyl-1-(3-iodopropargyl)-pyrrolidinium 4-methylbenzenesulfonate 1-[2-(decyloxycarbonyl)]ethyl-1-(3-iodopropargyl)-pyrrolidinium methanesulfonate. 